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1WTD

Crystal structure of type II restrcition endonuclease, EcoO109I DNA-free form

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL38B1
Synchrotron siteSPring-8
BeamlineBL38B1
Temperature [K]100
Detector technologyCCD
DetectorADSC QUANTUM 4
Wavelength(s)1.0000, 1.0082, 1.0089, 0.9
Spacegroup nameI 4
Unit cell lengths175.551, 175.551, 44.603
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 2.401
R-factor0.257
Rwork0.255
R-free0.29700
Structure solution methodMAD
RMSD bond length0.014
RMSD bond angle1.426
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareSOLVE
Refinement softwareREFMAC (5.2.0005)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.490
High resolution limit [Å]2.4002.400
Number of reflections26765
Completeness [%]99.199
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8293PEG 4000, potassium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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