1WT6

Coiled-Coil domain of DMPK

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98, 0.9793, 0.9795, 0.9392
Spacegroup name	P 21 21 21
Unit cell lengths	39.083, 46.164, 143.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.600
R-factor	0.23014
Rwork	0.228
R-free	0.27098
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.717
Data scaling software	XDS
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	32044
<I/σ(I)>	15
Completeness [%]	96.3	79.2
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100mM Tris-HCl pH 7.5, 2.0M Na+/K+ tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K