1WST

Crystal structure of multiple substrate aminotransferase (MsAT) from Thermococcus profundus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	90
Detector technology	CCD
Collection date	2004-06-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.978
Spacegroup name	C 2 2 21
Unit cell lengths	73.930, 117.780, 115.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.017 - 1.950
Rwork	0.189
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.366
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	2.060
High resolution limit [Å]	1.950	1.950
Number of reflections	37242
Completeness [%]	100.0	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	PEG 4000, isopropanol, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K