1WSA

STRUCTURE OF L-ASPARAGINASE II PRECURSOR

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-06-08
Detector	RIGAKU
Spacegroup name	P 21 21 2
Unit cell lengths	113.600, 85.500, 71.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.158
Rwork	0.158
RMSD bond length	0.015
RMSD bond angle	24.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Rmerge	0.102
Number of reflections	40359
Completeness [%]	84.6
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	61-68 (%)
2	1	reservoir	sodium phosphate	10 (mM)