1WS8

Crystal Structure of Mavicyanin from Cucurbita pepo medullosa (Zucchini)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	2002-10-10
Detector	WEISSENBERG
Wavelength(s)	1.000
Spacegroup name	P 61
Unit cell lengths	63.920, 63.920, 245.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.720 - 1.600
R-factor	0.193
Rwork	0.193
R-free	0.21100
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.038	0.175
Number of reflections	71278
<I/σ(I)>	13	13
Completeness [%]	86.0	81.3
Redundancy	93.2	81.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	ammonium sulfate, sodium cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	ammonium sulfate, sodium cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K