1WS6
The Structure of Thermus thermphillus HB8 hypothetical protein TTHA0928
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 174 |
| Detector technology | CCD |
| Collection date | 2003-02-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.88, 0.9795, 0.9795, 1.03 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.760, 58.760, 101.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.222 |
| Rwork | 0.218 |
| R-free | 0.28300 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.274 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.077 | 0.335 |
| Number of reflections | 7434 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 278 | PEG 10000, potassium thiocyanate, pH 6.5, micro-batch, temperature 278K |
