1WS2
urate oxidase from aspergillus flavus complexed with 5,6-diaminouracil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 140.181, 140.181, 151.176 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| Rwork | 0.163 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r51 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.450 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.100 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 48554 |
| <I/σ(I)> | 15.4 |
| Completeness [%] | 99.9 |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
