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1WRB

Crystal structure of the N-terminal RecA-like domain of DjVLGB, a pranarian Vasa-like RNA helicase

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL44B2
Synchrotron siteSPring-8
BeamlineBL44B2
Temperature [K]100
Detector technologyCCD
Collection date2002-12-01
DetectorMARRESEARCH
Wavelength(s)1.00
Spacegroup nameP 41 21 2
Unit cell lengths138.242, 138.242, 47.508
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution50.000 - 2.400
Rwork0.241
R-free0.28150
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle1.358
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.440
High resolution limit [Å]2.4002.400
Number of reflections18468
Completeness [%]98.994.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5293MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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