1WR8
Crystal structure of hypothetical protein PH1421 from Pyrococcus horikoshii.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-04 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0000, 1.590996, 1.591394, 1.594101 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.795, 81.772, 86.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 1.600 |
| R-factor | 0.1973 |
| Rwork | 0.197 |
| R-free | 0.21170 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.043 | 0.296 |
| Number of reflections | 59382 | |
| <I/σ(I)> | 19.1 | 6.2 |
| Completeness [%] | 98.2 | 96.9 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 291 | ammonium acetate, sodium acetate, PEG 4000, glycerol, potassium dihydrogen phosphate, pH 4.6, MICROBATCH, temperature 291K |
