1WQA
Crystal Structure of Pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.96410 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.653, 137.916, 98.409 |
| Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.216 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k2y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.242 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | 0.355 |
| Number of reflections | 139165 | |
| <I/σ(I)> | 8.3 | 1.85 |
| Completeness [%] | 95.3 | 82.8 |
| Redundancy | 2.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 6000, lithium chloride, tris(hydroxymethyl)aminomethane, glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
