1WQ7
Crystal Structure Of Biotin-(Acetyl-CoA-Carboxylase) ligase From Pyrococcus Horikoshii Ot3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-14 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0, 0.97934, 0.97972, 0.9650 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.601, 78.264, 70.147 |
| Unit cell angles | 90.00, 101.48, 90.00 |
Refinement procedure
| Resolution | 39.130 - 1.600 |
| Rwork | 0.216 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.061 | 0.371 |
| Number of reflections | 50181 | |
| <I/σ(I)> | 13.4 | 3.74 |
| Completeness [%] | 93.1 | 87.9 |
| Redundancy | 3.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 295 | PEG20K, Acetate, NaOH, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
