1WPM
Structure of Bacillus subtilis inorganic pyrophosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.812 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.675, 117.293, 144.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.050 |
| R-factor | 0.19216 |
| Rwork | 0.189 |
| R-free | 0.24803 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.533 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.080 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 64380 | |
| Completeness [%] | 98.8 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | hepes, ammonium sulphate, PEG-400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
