1WPB
Structure of Escherichia coli yfbU gene product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 230.521, 230.521, 230.521 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.1897 |
| Rwork | 0.190 |
| R-free | 0.22652 |
| Structure solution method | SIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.620 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 | 0.951 |
| Number of reflections | 272350 | |
| <I/σ(I)> | 28.6 | 1.91 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10.9 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | sodium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
