1WPA

1.5 Angstrom crystal structure of human occludin fragment 413-522

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-22
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	33.020, 35.310, 107.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.500
R-factor	0.24
Rwork	0.237
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.580
High resolution limit [Å]	1.500	1.500
Number of reflections	20588
Completeness [%]	97.9	90.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.6	293	ammonium sulfate, pH 5.6, EVAPORATION, temperature 293K
1	EVAPORATION	5.6	293	ammonium sulfate, pH 5.6, EVAPORATION, temperature 293K