1WP9
Crystal structure of Pyrococcus furiosus Hef helicase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.25 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.498, 165.940, 119.157 |
| Unit cell angles | 90.00, 114.85, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.28600 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.389 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.069 | 0.482 |
| Number of reflections | 92430 | |
| Completeness [%] | 99.3 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | 10% PEG 6000, 100mM Tricine-Na, 150mM NaCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
