1WOV
Crystal structure of heme oxygenase-2 from Synechocystis sp. PCC 6803 in complex with heme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.063 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.163, 74.585, 72.661 |
Unit cell angles | 90.00, 108.15, 90.00 |
Refinement procedure
Resolution | 19.760 - 1.750 |
R-factor | 0.19818 |
Rwork | 0.193 |
R-free | 0.25374 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dve |
RMSD bond length | 0.024 |
RMSD bond angle | 1.871 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 53138 | |
Completeness [%] | 89.2 | 93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | pottasium sodium tartrate, pottasium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |