1WOS

Crystal Structure of T-protein of the Glycine Cleavage System

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98020, 0.97947, 0.97935, 0.9500
Spacegroup name	P 21 21 21
Unit cell lengths	52.365, 53.852, 149.157
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 1.840
R-factor	0.218
Rwork	0.218
R-free	0.24800
Structure solution method	Crystal 1 SINGLE WAVELENGTH PROTOCOL, Crysatl 2 MAD PROTOCOL
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.910
High resolution limit [Å]	1.840	1.840
Number of reflections	37551
Completeness [%]	98.6	90

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.25	297	PEG 3350, sodium dihydrogen phosphate, pH 4.25, VAPOR DIFFUSION, HANGING DROP, temperature 297K
1	VAPOR DIFFUSION, HANGING DROP	4.25	297	PEG 3350, sodium dihydrogen phosphate, pH 4.25, VAPOR DIFFUSION, HANGING DROP, temperature 297K