1WOR

Crystal Structure of T-protein of the Glycine Cleavage System

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	52.435, 53.955, 149.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.980 - 1.950
R-factor	0.219
Rwork	0.219
R-free	0.24400
Structure solution method	Crystal 1 SINGLE WAVELENGTH PROTOCOL, Crysatl 2 MAD PROTOCOL
RMSD bond length	0.057
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.010
High resolution limit [Å]	1.950	1.950
Number of reflections	32274
Completeness [%]	98.7	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.25	297	PEG 3350, sodium dihydrogen phosphate, pH 4.25, VAPOR DIFFUSION, HANGING DROP, temperature 297K
1	VAPOR DIFFUSION, HANGING DROP	4.25	297	PEG 3350, sodium dihydrogen phosphate, pH 4.25, VAPOR DIFFUSION, HANGING DROP, temperature 297K