1WOL
Crystal Structure of ST0689, an archaeal HEPN homologue
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9765, 0.97894, 0.97924 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 62.553, 62.553, 64.010 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.350 - 1.620 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.22200 |
Structure solution method | MAD |
RMSD bond length | 0.041 |
RMSD bond angle | 3.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.620 | 1.620 |
Completeness [%] | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | tert-butanol, citrate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |