1WNS

Crystal structure of family B DNA polymerase from hyperthermophilic archaeon pyrococcus kodakaraensis KOD1

Replaces:  1GCX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	1999-02-26
Detector	WEISSENBERG
Spacegroup name	P 21 21 21
Unit cell lengths	72.640, 111.380, 111.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.240 - 3.000
R-factor	0.231
Rwork	0.231
R-free	0.31300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.100
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.084	0.345
Number of reflections	20298
<I/σ(I)>	8.7
Completeness [%]	88.1	83.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	MPD, pH 5.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K