1WNF

Crystal Structure of PH0066 from Pyrococcus horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-04
Detector	RIGAKU JUPITER
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	125.230, 77.140, 98.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.230 - 2.500
R-factor	0.199
Rwork	0.199
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nns
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.230	2.590
High resolution limit [Å]	2.300	2.500
Number of reflections	33612
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.1	298	PEG 2000, CHES-HCl, pH 9.1, VAPOR DIFFUSION, temperature 298K