1WN6

Crystal Structure of Blasticidin S Deaminase (BSD) Complexed with Tetrahedral Intermediate of Blasticidin S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.02
Spacegroup name	P 43 21 2
Unit cell lengths	54.690, 54.690, 197.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.640 - 1.800
R-factor	0.219
Rwork	0.218
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.850
High resolution limit [Å]	1.800
Rmerge	0.072
Number of reflections	28550
Completeness [%]	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1