1WN0

Crystal Structure of Histidine-containing Phosphotransfer Protein, ZmHP2, from maize

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-28
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	148.800, 81.410, 89.500
Unit cell angles	90.00, 123.42, 90.00

Refinement procedure

Resolution	19.840 - 2.200
R-factor	0.211
Rwork	0.209
R-free	0.24800
Structure solution method	MAD
Starting model (for MR)	selenomethionine labeled protein
RMSD bond length	0.041
RMSD bond angle	2.556
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	42707
Completeness [%]	94.1	91.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	50mM ammonium sulfate, 100mM sodium acetate buffer, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	50mM ammonium sulfate, 100mM sodium acetate buffer, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	50mM ammonium sulfate, 100mM sodium acetate buffer, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K