1WMY
Crystal Structure of C-type Lectin CEL-I from Cucumaria echinata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.380, 69.940, 76.690 |
| Unit cell angles | 90.00, 136.46, 90.00 |
Refinement procedure
| Resolution | 52.700 - 2.000 |
| R-factor | 0.14016 |
| Rwork | 0.138 |
| R-free | 0.17920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lit |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.702 |
| Data reduction software | DPS |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 20936 | |
| Completeness [%] | 92.3 | 82.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 60% 2-methyl-2,4-pentanediol, 10mM calcium chloride, 0.1M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
