1WMW
Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-02-28 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.97880, 0.97777, 0.97760, 0.96860 |
Spacegroup name | P 43 |
Unit cell lengths | 139.880, 139.880, 73.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.980 - 1.550 |
R-factor | 0.175 |
Rwork | 0.175 |
R-free | 0.19800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.039 | 0.115 |
Number of reflections | 182752 | |
Completeness [%] | 89.2 | 81.8 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 296 | PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K |