1WMO
Crystal structure of topaquinone-containing amine oxidase activated by nickel ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.7 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 157.679, 62.993, 183.670 |
| Unit cell angles | 90.00, 111.83, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.810 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.305 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.124 | |
| Number of reflections | 214460 | |
| Completeness [%] | 98.3 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.8 | 289 | 1.05M POTASSIUM SODIUM TARTRATE, 25mM HEPES, pH 6.8, MICRODIALYSIS, temperature 289K |
