1WMI
Crystal structure of archaeal RelE-RelB complex from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9794, 0.9792, 0.9000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.533, 142.651, 37.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| Rwork | 0.228 |
| R-free | 0.27600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.137 |
| Number of reflections | 11137 | |
| <I/σ(I)> | 24.3 | |
| Completeness [%] | 93.1 | 72.3 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.4 | tri-Ammonium citrate, MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.4K |
