1WMH
Crystal structure of a PB1 domain complex of Protein kinase c iota and Par6 alpha
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791, 0.9793, 0.9795, 0.9843 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 94.150, 38.363, 45.478 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.530 - 1.500 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.22400 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.206 |
| Number of reflections | 50330 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 98.5 | 99.3 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | sodium formate, Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
