1WMG

Crystal structure of the UNC5H2 death domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-06
Detector	RIGAKU JUPITER
Wavelength(s)	0.979006, 0.979363, 0.964000
Spacegroup name	C 1 2 1
Unit cell lengths	124.878, 47.207, 121.177
Unit cell angles	90.00, 119.56, 90.00

Refinement procedure

Resolution	39.780 - 2.100
R-factor	0.229
Rwork	0.229
R-free	0.26900
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	71778
Completeness [%]	99.6	99.2
Redundancy	3.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	6.5	285	MES, magnesium sulfate, pH 6.5, LIQUID DIFFUSION, temperature 285K