1WMF
Crystal Structure of alkaline serine protease KP-43 from Bacillus sp. KSM-KP43 (oxidized form, 1.73 angstrom)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-24 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 42.917, 109.153, 167.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.730 |
R-factor | 0.142 |
R-free | 0.21160 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 0.024 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.790 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.057 | 0.204 |
Number of reflections | 40078 | |
<I/σ(I)> | 21.98 | 6.086 |
Completeness [%] | 96.4 | 86.6 |
Redundancy | 5.761 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 0.1M HEPES-NaOH pH 7.3, 1.7M ammonium sulfate, 10mM calcium chloride, 10% (v/v) 1,4-dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 293K |