1WMF
Crystal Structure of alkaline serine protease KP-43 from Bacillus sp. KSM-KP43 (oxidized form, 1.73 angstrom)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-24 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.917, 109.153, 167.164 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.730 |
| R-factor | 0.142 |
| R-free | 0.21160 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.057 | 0.204 |
| Number of reflections | 40078 | |
| <I/σ(I)> | 21.98 | 6.086 |
| Completeness [%] | 96.4 | 86.6 |
| Redundancy | 5.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 0.1M HEPES-NaOH pH 7.3, 1.7M ammonium sulfate, 10mM calcium chloride, 10% (v/v) 1,4-dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
