1WLZ
Crystal structure of DJBP fragment which was obtained by limited proteolysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.671, 68.519, 69.208 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.600 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.22300 |
| Structure solution method | MAD+SIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.042 | |
| Number of reflections | 51566 | |
| Completeness [%] | 99.4 | 95.1 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30% PEG MME 2000, 0.1M Na Acetate pH 4.6, 0.2M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
