1WLY
Crystal Structure of 2-Haloacrylate Reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.797, 49.176, 59.053 |
Unit cell angles | 90.00, 100.97, 90.00 |
Refinement procedure
Resolution | 19.330 - 1.300 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.23200 |
Structure solution method | MIR |
RMSD bond length | 0.027 |
RMSD bond angle | 2.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.380 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 94850 | |
Completeness [%] | 97.2 | 90.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |