1WLI
L122Y mutant of FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-07 |
Detector | OXFORD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.673, 79.391, 39.970 |
Unit cell angles | 90.00, 91.25, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.600 |
R-factor | 0.153 |
Rwork | 0.153 |
R-free | 0.18500 |
Structure solution method | AB INITIO |
Starting model (for MR) | 1flm |
RMSD bond length | 0.009 |
RMSD bond angle | 0.023 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXL-97 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 1.660 | |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 29692 | |
Completeness [%] | 98.0 | 84.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG6000, sodium acetate, Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |