1WLH
Molecular structure of the rod domain of Dictyostelium filamin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.320, 61.670, 119.030 |
| Unit cell angles | 90.00, 104.10, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.25775 |
| Rwork | 0.257 |
| R-free | 0.26315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ksr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.677 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.026 | 0.124 |
| Number of reflections | 20708 | |
| Completeness [%] | 99.3 | 90.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | MPD, calcium chloride, sodium acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
