1WLC

Congerin II Y16S/T88I double mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-09-17
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 42 21 2
Unit cell lengths	60.830, 60.830, 79.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
Rwork	0.182
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.000
Rmerge	0.058
Number of reflections	10418
Completeness [%]	95.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.8M magnesium sulfate, 0.05M Mes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K