1WL5
Human cytosolic acetoacetyl-CoA thiolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 57.850, 109.880, 155.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.260 |
| R-factor | 0.14432 |
| Rwork | 0.142 |
| R-free | 0.19420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CoA complex |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.460 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 23254 | |
| <I/σ(I)> | 11.1 | 3.4 |
| Completeness [%] | 98.2 | 99.3 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | ammonium sulfate, lithium sulfate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
