1WKV
Crystal structure of O-phosphoserine sulfhydrylase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9797 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.874, 74.874, 275.908 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.000 - 2.000 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.23100 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.521 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.063 | 0.278 |
Number of reflections | 52917 | |
<I/σ(I)> | 11 | |
Completeness [%] | 97.3 | 82.4 |
Redundancy | 6.4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | pyridoxal 5'-phosphate, 2-mercaptoethanol, cacodylate, sodium acetate, PEG 8000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |