1WKU

High resolution structure of the human alpha-actinin isoform 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	38.499, 45.960, 66.998
Unit cell angles	86.67, 87.80, 87.33

Refinement procedure

Resolution	33.420 - 1.600
R-factor	0.182
Rwork	0.182
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	LOW RESOLUTION MODEL OF DIFFERENT SPACE GROUP
RMSD bond length	0.004
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.420	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	56760
Completeness [%]	94.0	87.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	peg 20000, sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K