1WKO
Terminal flower 1 (tfl1) from arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 109.347, 109.347, 64.697 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.1855 |
| Rwork | 0.184 |
| R-free | 0.20672 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qou |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.539 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.110 |
| Number of reflections | 41659 |
| <I/σ(I)> | 20.1 |
| Completeness [%] | 100.0 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.3M (NH4)2SO4, 22% w/v PEG 5000 MME, 0.1M cacodylate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
