1WKG

Acetylornithine aminotransferase from thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	180
Detector technology	IMAGE PLATE
Collection date	2003-07-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	71.270, 69.790, 140.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.250
Rwork	0.226
R-free	0.28880
Structure solution method	MOLECULAR REPLACEMENT
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.050
Number of reflections	261469
Completeness [%]	95.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K