1WKC

Crystal structure of a 5-formyltetrahydrofolate cycloligase-related protein from Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-20
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.97923, 0.97954, 0.90000
Spacegroup name	I 2 2 2
Unit cell lengths	54.010, 73.370, 107.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 1.700
R-factor	0.21995
Rwork	0.218
R-free	0.25082
Structure solution method	MAD
RMSD bond length	0.022
RMSD bond angle	2.028
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.860	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.043	0.264
Number of reflections	23751
<I/σ(I)>	21.7
Completeness [%]	99.6	100
Redundancy	7.04	7.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1