1WJG
Crystal structure of a probable ATP binding protein from thermus themophilus HB8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-04 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97898, 0.97927, 0.98200 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.100, 72.100, 54.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.247 |
Rwork | 0.247 |
R-free | 0.26300 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.400 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.059 | 0.320 |
Number of reflections | 11879 | |
<I/σ(I)> | 43.6 | 9.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.5 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 20% PEG200, 0.1M MES, 0.04M Strontium chloride, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |