1WJ9
Crystal structure of a CRISPR-associated protein from thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-26 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 |
Unit cell lengths | 76.673, 76.673, 87.494 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.340 - 1.900 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.24700 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.039 | 0.279 |
<I/σ(I)> | 35.6 | 3.4 |
Completeness [%] | 98.1 | 97.8 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 20% PEGMME2000, 0.1M bicine, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 20% PEGMME2000, 0.1M bicine, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |