1WIQ

STRUCTURE OF T-CELL SURFACE GLYCOPROTEIN CD4, TRIGONAL CRYSTAL FORM

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	298
Spacegroup name	P 31 2 1
Unit cell lengths	126.000, 126.000, 205.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 5.000
R-factor	0.422
Rwork	0.422
R-free	0.44200
Structure solution method	MOLECULAR REPLACEMENT PLUS ANOMALOUS DIFFRACTION.
RMSD bond length	0.015
RMSD bond angle	2.300
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	5.000 *
Rmerge	0.086 *	0.295 *
Number of reflections	4289 *
Completeness [%]	67.3 *	49.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2 *	20 *	Kwong, P.D., (1990) Proc. Natl. Acad. Sci. U.S.A., 87, 6423. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG400	0.23 (%)
2	1	drop	ammonium phosphate	183 (mM)
3	1	drop	sodium phosphate	40 (mM)
4	1	drop	Tris-HCl	10 (mM)
5	1	drop		3.1 (mM)
6	1	drop	sCD4	4.0 (mg/ml)
7	1	reservoir	ammonium sulfate	39.0 (%sat)
8	1	reservoir	Tris-HCl	100 (mM)
9	1	reservoir		74 (mM)