1WIP

STRUCTURE OF T-CELL SURFACE GLYCOPROTEIN CD4, MONOCLINIC CRYSTAL FORM

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	P 1 2 1
Unit cell lengths	100.500, 123.400, 100.600
Unit cell angles	90.00, 103.40, 90.00

Refinement procedure

Resolution	8.000 - 4.000
R-factor	0.452
Rwork	0.452
R-free	0.42700
Structure solution method	MOLECULAR REPLACEMENT PLUS ANOMALOUS DIFFRACTION.
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REPLACE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	4.000 *
Rmerge	0.062 *	0.254 *
Number of reflections	13474 *
Completeness [%]	76.0 *	45.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	4 *	Kwong, P.D., (1990) Proc. Natl. Acad. Sci. U.S.A., 87, 6423. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG400	2.0 (%)
2	1	drop	ammonium sulfate	15 (mM)
3	1	drop	sodium cacodylate	6.0 (mM)
4	1	drop	Tris-HCl	5.0 (mM)
5	1	drop	sCD4	3.1 (mg/ml)
6	1	reservoir	PEG400	30.0 (%)
7	1	reservoir	Tris-HCl	100 (mM)
8	1	reservoir		29.6 (mM)