1WIO
STRUCTURE OF T-CELL SURFACE GLYCOPROTEIN CD4, TETRAGONAL CRYSTAL FORM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 127.100, 127.100, 221.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.900 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.35100 |
| Structure solution method | MOLECULAR REPLACEMENT PLUS ANOMALOUS DIFFRACTION. |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 * | |
| High resolution limit [Å] | 3.900 * | |
| Rmerge | 0.112 * | 0.340 * |
| Number of reflections | 11993 * | |
| Completeness [%] | 80.0 * | 53.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * | Kwong, P.D., (1990) Proc. Natl. Acad. Sci. U.S.A., 87, 6423. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG400 | 1.4 (%) | |
| 2 | 1 | drop | ammonium sulfate | 10.7 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 4.3 (mM) | |
| 4 | 1 | drop | Tris-HCl | 3.6 (mM) | |
| 5 | 1 | drop | 1.2 (mM) | ||
| 6 | 1 | drop | sCD4 | 2.2 (mg/ml) | |
| 7 | 1 | reservoir | PEG400 | 40.0 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 9 | 1 | reservoir | 49.3 (mM) |
