1WIO

STRUCTURE OF T-CELL SURFACE GLYCOPROTEIN CD4, TETRAGONAL CRYSTAL FORM

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	P 43 2 2
Unit cell lengths	127.100, 127.100, 221.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.900
R-factor	0.24
Rwork	0.240
R-free	0.35100
Structure solution method	MOLECULAR REPLACEMENT PLUS ANOMALOUS DIFFRACTION.
RMSD bond length	0.015
RMSD bond angle	2.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	3.900 *
Rmerge	0.112 *	0.340 *
Number of reflections	11993 *
Completeness [%]	80.0 *	53.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	20 *	Kwong, P.D., (1990) Proc. Natl. Acad. Sci. U.S.A., 87, 6423. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG400	1.4 (%)
2	1	drop	ammonium sulfate	10.7 (mM)
3	1	drop	sodium cacodylate	4.3 (mM)
4	1	drop	Tris-HCl	3.6 (mM)
5	1	drop		1.2 (mM)
6	1	drop	sCD4	2.2 (mg/ml)
7	1	reservoir	PEG400	40.0 (%)
8	1	reservoir	Tris-HCl	100 (mM)
9	1	reservoir		49.3 (mM)