1WFB
WINTER FLOUNDER ANTIFREEZE PROTEIN ISOFORM HPLC6 AT-180 DEGREES C
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1993-07 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.409, 36.543, 21.360 |
| Unit cell angles | 90.00, 103.36, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.500 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.19600 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.800 |
| Data reduction software | RIGAKU |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 * |
| High resolution limit [Å] | 1.500 * |
| Rmerge | 0.054 |
| Total number of observations | 28251 * |
| Number of reflections | 8329 |
| Completeness [%] | 89.2 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.3 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | acetone | ||
| 2 | 1 | 1 | protein | 4 (mg/ml) | |
| 3 | 1 | 1 | Tris-HCl | 20 (mM) |
