1WFA

WINTER FLOUNDER ANTIFREEZE PROTEIN ISOFORM HPLC6 AT 4 DEGREES C

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-07
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	38.170, 37.040, 21.890
Unit cell angles	90.00, 101.14, 90.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.179
Rwork	0.179
R-free	0.22600
RMSD bond length	0.014
RMSD bond angle	2.200
Data reduction software	RIGAKU
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	1.700 *
Rmerge	0.044
Total number of observations	16614 *
Number of reflections	5505
Completeness [%]	81.7
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.3 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	acetone
2	1	1	protein	4 (mg/ml)
3	1	1	Tris-HCl	20 (mM)