1WET
STRUCTURE OF THE PURR-GUANINE-PURF OPERATOR COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | AREA DETECTOR |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 175.990, 95.080, 81.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.600 |
| R-factor | 0.176 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.260 |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.700 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.063 | |
| <I/σ(I)> | 7.4 | 1.4 |
| Completeness [%] | 87.0 | 78 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | Schumacher, M.A., (1994) J.Mol.Biol, 242, 302. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | NH4 PHOSPHATE | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 1 | NH4 SULFATE | ||
| 4 | 1 | 1 | [CO(NH3)6]CL3 | ||
| 5 | 1 | 1 | NH4 PHOSPHATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | PEG 4000 | ||
| 8 | 1 | 2 | NH4 SULFATE | ||
| 9 | 1 | 2 | [CO(NH3)6]CL3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.5 (mM) | |
| 10 | 1 | reservoir | PEG4000 | 25 (%) | |
| 11 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
| 12 | 1 | reservoir | cobalt hexammine | 50 (mM) | |
| 13 | 1 | reservoir | ammonium phosphate | 0.1 (M) | |
| 2 | 1 | drop | DTT | 1.0 (mM) | |
| 3 | 1 | drop | sodium potassium phosphate | 160 (mM) | |
| 4 | 1 | drop | DNA | 0.6 (mM) | |
| 5 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 6 | 1 | drop | PEG4000 | 25 (%) | |
| 7 | 1 | drop | ammonium sulfate | 0.4 (M) | |
| 8 | 1 | drop | cobalt hexammine | 50 (mM) | |
| 9 | 1 | drop | ammonium phosphate | 0.1 (M) |
