1WEI
Catalytic Domain Of Muty From Escherichia Coli K20A Mutant Complexed To Adenine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.975913 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.502, 49.325, 69.609 |
| Unit cell angles | 90.00, 122.59, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.450 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 37704 | |
| Completeness [%] | 87.1 | 56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
